Two Spirit or Two-Spirited is an exclusive identity for Indigenous North Americans and is defined as an umbrella term for sexuality, gender and/or spirituality. Two Spirit being seen as an umbrella term encompasses homosexual/homoromantic attraction and a wide variety of gender variance that includes individuals who in western culture may be described as gay, lesbian, bi, trans, genderqueer, GNC or those who have more than one gender identity. Two-Spirit also may include relationships which are non-monogamous.

Two-spirited individuals often serve a ceremonial role as well. In some tribes, when the term is used as an English synonym for a traditional ceremonial role, a two-spirited individual must be recognized as such by the Elders of their community. Most tribes have a tribe-specific name that is used to describe a third or fourth gender category, and may be used synonymously with two spirit in English. Two spirit was created as an English word to serve as an umbrella term for the diverse, culturally-specific ceremonial and social roles found in many different Native American and Indigenous cultures. The roles that can be described as two spirit can vary widely. No single gender or sexuality category within Native American/Indigenous' cultures applies to all two spirit individuals and vice verse.

As an umbrella term it can be more accurately understood as any identity that does not make sense unless it is contextualized within the framework of a Native American or Indigenous traditional cultural understanding. Not all two-spirited individuals consider themselves transgender, non-binary, or LGBT+ however some two-spirited individuals do seek to be included under LGBTQIA2+, as there were Indigenous Native individuals fighting for gay rights at the Stonewall riot in 1969.